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. 2022 Jul 7;12(2):1602–1615. doi: 10.1002/cam4.4970

FIGURE 2.

FIGURE 2

IMPA1 promotes TNBC cells proliferation, colony formation in vitro. (A) SUM159PT and BT549 cells stably expressing pLVX and HA‐IMPA1 were verified using immunoblotting with the corresponding antibodies. (B–D) SUM159PT and BT549 cells stably expressing pLVX and HA‐IMPA1 were subjected to cell proliferation assays using CCK‐8 kit (B), and colony formation assays (C), and the corresponding quantitative results (D) are shown. (E) LM2‐4175, BT549, and MDA‐MB‐231 cells stably expressing shNC and shIMPA1 were verified using immunoblotting with the corresponding antibodies. (F–H) LM2‐4175, BT549, and MDA‐MB‐231 cells stably expressing shNC and shIMPA1 were used to investigate cell proliferation by CCK‐8 assays (F) and colony formation assays (G) and the corresponding quantitative results (H) are shown. (I) Verification of reverted IMPA1 expression after knockdown in MDA‐MB‐231 cells. (J–L) MDA‐MB‐231 cells with reverted IMPA1 expression were used to detect cell proliferation by CCK‐8 assays (J) and colony formation assays (K) and the corresponding quantitative results (L) are shown. (M) Cell‐cycle distribution was analyzed by flow cytometric assays in LM2‐4175 and MDA‐MB‐231 cells stably expressing shNC and shIMPA1 and the corresponding quantitative results are shown. p < 0.05 was considered statistically significant.