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. Author manuscript; available in PMC: 2023 Jan 28.
Published in final edited form as: Cell Rep. 2022 Nov 8;41(6):111628. doi: 10.1016/j.celrep.2022.111628

Figure 2. Serum and IgG+ B cell analysis of rsHAtCh immunization.

Figure 2.

(A) Schematic representation of immunization regimen in IGHV1–2 HC2 mice. Serum harvested 2 weeks after final immunization. Two replicates of each cohort immunized.

(B) Serum ELISA titers against H1 SI-06 FLsE and H1 SI-06 ΔRBS FLsE, where the RBS is sterically occluded by an N-linked glycan a position 190, for control cohort (N = 10) and rsHAtCh cohort (N = 8) mice. Statistical significance was determined using the Mann-Whitney U test (***p < 0.001).

(C) rsHAtCh cohort serum ELISA titers against five historical H1 FLsEs. Statistical significance was determined using the Mann-Whitney U test (***p < 0.001).

(D) Frequency of class-switched IgG+ B cells engaging RBS (ΔRBS non-binders) or non-RBS epitopes (ΔRBS binders); total numbers of cells sorted pooled across each cohort listed.

Related to Figure S2.