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. Author manuscript; available in PMC: 2023 Jan 28.
Published in final edited form as: Cell Rep. 2022 Nov 8;41(6):111628. doi: 10.1016/j.celrep.2022.111628

Figure 6. rsHAtCh-elicited RBS-directed antibodies neutralize strain-matched virus and partially protect.

Figure 6.

(A) Microneutralization titers of seven antibodies tested against a panel of eight historical H1 virus isolates ranging from 1934 to 2009. Titers are reported as the maximal concentration (ug/mL) of each antibody at which complete neutralization was observed.

(B) Cartoon representation of Ab109 footprint on HA; heavy-chains contact shown in dark blue, both heavy- and light-chain contacts shown in light blue, and light-chain contacts shown in cyan. CDR L1, L3, and H3 loops are labeled. Residue changes across historical H1 isolates expected to abrogate binding shown in red, labeled with key mutations found in either H1 MA-90, H1 FL-93, H1 BE-95, or H1 CA-09.

(C) Protection studies following NC-99 infection in cDC-IrF5−/− knockout mice (N = 5 PBS control, N = 6 Ab111 cohorts). The y axes show percentage of weight change from initial weight (morbidity, left) and percentage of survival (mortality, right); *p < 0.01 compared with PBS control using the Mantle-Cox test.