Table 1.
Breadth of rsHAtCh-elicited RBS-directed antibodies
| USSR77 | KW86 | MA90 | FL93 | BE95 | NC99 | SI06 | CA09 | rsH3 | rsH4 | rsH14 | |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Ab100 | >100 | >100 | >100 | >100 | >100 | 0.76 | 0.82 | >100 | 1.2 | 1.2 | 0.10 |
| Ab102 | >100 | >100 | >100 | >100 | >100 | <0.02 | <0.02 | >100 | 0.05 | 0.14 | <0.02 |
| Ab104 | >100 | >100 | >100 | >100 | >100 | >100 | >100 | >100 | >100 | >100 | >100 |
| Ab105 | >100 | >100 | >100 | >100 | >100 | 0.19 | 0.19 | >100 | 0.57 | 3.9 | 0.22 |
| Ab108 | >100 | >100 | >100 | >100 | >100 | 0.23 | 0.16 | >100 | 0.38 | 0.93 | 0.05 |
| Ab109 | >100 | >100 | 48 | >100 | >100 | 0.04 | 0.03 | >100 | 0.10 | 0.57 | 0.03 |
| Ab110 | >100 | >100 | >100 | >100 | >100 | 0.04 | 0.06 | >100 | 0.14 | 0.38 | 0.04 |
| Ab111 | >100 | >100 | >100 | >100 | >100 | 0.03 | 0.02 | >100 | 0.06 | 0.62 | 0.02 |
| Ab114 | >100 | >100 | >100 | 22 | >100 | 0.06 | 0.49 | >100 | 0.43 | 1.1 | <0.02 |
| Ab116 | >100 | >100 | >100 | >100 | >100 | 1.1 | 2.8 | >100 | 1.0 | 2.0 | 0.11 |
| Ab117 | >100 | >100 | >100 | >100 | >100 | 0.12 | 0.12 | >100 | 0.47 | 1.0 | 0.17 |
| Lin100 UCA | N.D. | N.D. | >100 | >100 | >100 | >100 | >100 | N.D. | >100 | >100 | >100 |
| Lin100 I-0 | N.D. | N.D. | >100 | >100 | >100 | 41 | >100 | N.D. | 30 | >100 | 11 |
| Lin114 UCA | N.D. | N.D. | >100 | >100 | >100 | 23 | 36 | N.D. | 23 | >100 | 5.8 |
| Lin119 UCA | N.D. | N.D. | >100 | >100 | >100 | 0.26 | 0.44 | N.D. | 0.69 | 1.5 | 0.09 |
| Lin140 UCA | N.D. | N.D. | >100 | >100 | >100 | 0.29 | 0.5 | N.D. | 0.67 | 1.6 | 0.1 |
| Lin143 UCA | N.D. | N.D. | >100 | >100 | >100 | 0.53 | 0.7 | N.D. | 0.67 | 6.8 | 0.37 |
rsHAtCh-elicited antibodies and computationally inferred lineage UCAs assayed for binding to a panel of historical H1s. KD values are for Fabs and monomeric HA heads expressed in μM. Lineages of clonally related mature antibodies labeled. N.D. is not determined, and >100 μM is beyond the limit of detection; Fabs with affinity are in bold for ease of viewing. At least four independent concentrations of analyte were used to obtain reported KD values. Related to Figure S5.