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. Author manuscript; available in PMC: 2023 Jan 28.
Published in final edited form as: Cell Rep. 2022 Nov 8;41(6):111628. doi: 10.1016/j.celrep.2022.111628

KEY RESOURCES TABLE

REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
HRP-conjugated rabbit anti-mouse IgG antibody Abcam CAT#ab97046
Ly-6G-APCe780 ThermoFisher CAT#14-5931-82
F4/80-APCe780 ThermoFisher CAT#47-4801-82
CD3-BV785 BioLegend CAT#100231
CD19-BV421 BioLegend CAT#115549
IgM-BV605 BioLegend CAT#406523
IgG-PerCP/Cy5.5 BioLegend CAT#405314
msIgG1 isoforms of Ab100, Ab102, Ab109, Ab110, Ab111, Ab114, Ab116 This paper N/A
Ab111 IgG2a isoform This paper N/A
Ab111 IgG2a LALA-PG isoform This paper N/A
Bacterial and virus strains
A/Michigan/45/2015 (H1N1) Duke RBL Lot 1483-180124
A/California/07/2009 (H1N1) Duke RBL Lot 13659-20141211
A/Solomon Islands/3/2006 (H1N1) Duke RBL Lot 331-05132014
A/Hawaii/15/2001 (H1N1) Duke RBL Lot FR-394-20200928
A/New Caledonia/20/1999 (H1N1) IRR / Duke RBL Lot FR-395-20200928
A/Beijing/262/1995 (H1N1) Duke RBL Lot 12277-16524
A/Texas/36/1991 Duke RBL Lot 1096-20200807
A/Puerto Rico/8/1934 Duke RBL Lot CR-05282014
Chemicals peptides and recombinant proteins
rsHAtCh (rsH4, rsH3, rsH14 heterotrimer) This paper N/A
rsH14 monomeric head domain Produced in house; Bajic et al.27 N/A
rsH3 monomeric head domain This paper N/A
rsH4 monomeric head domain Produced in house; Bajic et al.27 N/A
GABH heterodimeric coiled coil (acidic and basic strands) Produced in house
Keating et al.61 		N/A
H2227 Produced in house
Schmidt et al.56 		N/A
H2526 Produced in house
Schmidt et al.56 		N/A
CH67 Produced in house
Whittle et al.37 		N/A
641 I-9 Produced in house
Schmidt et al.56 		N/A
C05 Produced in house
Ekiert et al.47 		N/A
HRV 41.1 Produced in house
Schmidt et al.56 		N/A
Full length soluble ectodomains of H1 MA-90, H1 BE-95, H1 NC-99, H1 SI-06, and H1 CA-09 Produced in house N/A
H1 SI-06 FLsE ΔRBS Produced in house
Lingwood et al.39 		N/A
Monomeric HA head domains of H1 USSR-77, KW-86, MA-90, FL-93, BE-95, NC-99, SI-06, CA-09 Produced in house
Schmidt et al.56 		N/A
Ab100 (VH/VL sequence from IGHV1-2 HC2 mouse) This paper OP251202 / OP251203
Ab102 (VH/VL sequence from IGHV1-2 HC2 mouse) This paper OP251204 / OP251205
Ab104 (VH/VL sequence from IGHV1-2 HC2 mouse) This paper OP251206 / OP251207
Ab105 (VH/VL sequence from IGHV1-2 HC2 mouse) This paper OP251208 / OP251209
Ab108 (VH/VL sequence from IGHV1-2 HC2 mouse) This paper OP251210 / OP251211
Ab109 (VH/VL sequence from IGHV1-2 HC2 mouse) This paper OP251212 / OP251213
Ab110 (VH/VL sequence from IGHV1-2 HC2 mouse) This paper OP251214 / OP251215
Ab111 (VH/VL sequence from IGHV1-2 HC2 mouse) This paper OP251216 / OP251217
Ab114 (VH/VL sequence from IGHV1-2 HC2 mouse) This paper OP251218 / OP251219
Ab116 (VH/VL sequence from IGHV1-2 HC2 mouse) This paper OP251220 / OP251221
Ab117 (VH/VL sequence from IGHV1-2 HC2 mouse) This paper OP251222 / OP251223
Lin100 UCA (VH/VL sequence from IGHV1-2 HC2 mouse) This paper OP251224 / OP251225
Lin100 (VH/VL sequence from IGHV1-2 HC2 mouse)I-0 This paper OP251226 / OP251227
Lin114 UCA (VH/VL sequence from IGHV1-2 HC2 mouse) This paper OP251228 / OP251229
Lin119 (VH/VL sequence from IGHV1-2 HC2 mouse)UCA This paper OP251230 / OP251231
Lin140 (VH/VL sequence from IGHV1-2 HC2 mouse)UCA This paper OP251232 / OP251233
Lin143 (VH/VL sequence from IGHV1-2 HC2 mouse)UCA This paper OP251234 / OP251235
Pierce HRV 3C protease ThermoScientific CAT#88946
Sigma Adjuvant System SigmaAldrich CAT#S6322
Streptavidin-PE conjugate Invitrogen CAT#12-4317-87
Aqua Live/Dead amine-reactive dye ThermoFisher CAT#L34957
Calcein, AM, cell-permeant dye ThermoFisher CAT#C3100MP
Streptavidin-APC/Cy7 conjugate BioLegend CAT#405208
Streptavidin-BV650 conjugate BioLegend CAT#405232
StrepTactin-PE conjugate IBA Lifesciences Item#6-5000-001
StrepTactin-APC conjugate IBA Lifesciences Item#6-5010-001
RNaseOUT ThermoFisher CAT#10777019
TALON Metal Affinity Resin Takara CAT#635652
Pierce Protein G Agarose ThermoFisher CAT#20399
1-Step ABTS substrate ThermoFisher Prod#37615
Critical commercial assays
Ni-NTA Biosensors ForteBio Item#18-5101
SuperScript IV VILO MasterMix ThermoFisher CAT#11756050
MagMax mirVana Total RNA isolation kit ThermoFisher CAT#A27828
Deposited data
Electron density map, Ab109 and H1 SI-06 FLsE This paper EMDB: EMD-26605
Structure, Ab109 and H1 SI-06 FLsE This paper PDB: 7UMM
Electron density map, Ab111 and H1 SI-06 FLsE This paper EMDB: EMD-26604
Isolated B cell antibody sequences This paper GenBank: OP251202 - OP251235
Experimental models: Cell lines
Human: FreeStyle 293F Thermo Fisher Cat#R79007; RRID: CVCL_D603
Human: Expi293F Thermo Fisher Cat#A14527; RRID: CVCL_D615
Experimental models: Organisms/strains
cDC-IrF5−/− mice Yousif et al.41 N/A
IGHV1-2 HC2 mice Sangesland et al.30 N/A
Oligonucleotides
Murine B cell receptor sequencing primers Tiller et al.; Rohatgi et al.,62,63 N/A
Software and algorithms
FlowJo v10 TreeStar https://www.flowjo.com;
RRID: SCR_008520
Prism v9 GraphPad https://www.graphpad.com;
RRID: SCR_002798
IMGT International ImMunoGeneTics
Information System		 http://www.imgt.org;
RRID: SCR_012780
Cloanalyst Kepler et al.64 https://www.bu.edu/computationalimmunology/research/software/
RELION Scheres et al.65 https://relion.readthedocs.io/en/release-3.1/
Chimera Pettersen et al.66 https://www.cgl.ucsf.edu/chimera/
PHASER McCoy et al.67 http://www.phaser.cimr.cam.ac.uk/index.php/Phaser_Crystallographic_Software
PHENIX Adams et al.68 https://www.phenix-online.org/
COOT Emsley et al.69 http://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
PyMol Schrödinger http://www.pymol.org
MolProbity Chen et al.70 http://molprobity.biochem.duke.edu
XDS Kabsch et al.71 https://xds.mr.mpg.de
Other
Superdex 200 Increase 10/300 GL GE Healthcare Cytiva 28-9909-44