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. 2023 Jan 28;11(1):e01056. doi: 10.1002/prp2.1056

FIGURE 1.

FIGURE 1

Leukocyte‐depleted platelets produce 15‐HETrE. (A) Leukocyte‐depleted platelets were stained with antibodies specific to platelets (GPIbα) and leukocytes (CD45) and analyzed by flow cytometry to quantify the number of residual polymorphonuclear leukocytes (PMNs) (CD45‐positive, GPIbα‐negative cells) in each sample. (B) The levels of 12‐HETrE and 15‐HETrE were measured in the releasate of leukocyte‐depleted platelets (n = 3) treated with DGLA (10  μM). Data represent mean ± SD.