FIGURE 5.

15‐HETE and 15‐HETrE inhibit platelet activation via distinct mechanisms. (A) The lysates of platelets (3 × 10⁸/mL) (n = 3‐4) treated with forskolin, a direct adenylyl cyclase agonist, oxylipins (10 μM) or DMSO were separated on a 10% SDS‐PAGE gel and western blots were performed with antibodies to phospho‐ and total VASP. (B) Platelets were incubated with PPARα (GW6471; 10 μM; n = 3‐6), PPARβ (GW3787; 10 μM; n = 3‐6), or PPARγ (GW9662; 10 μM; n = 3‐6) antagonist, prior to the treatment with 15‐LOX oxylipin, and then stimulated with collagen (0.25‐1 μg/mL). Data represent mean ± SEM. Two‐tailed paired t test. DMSO concentration corresponds to the residual DMSO in the 10 μM oxylipin sample.