TABLE 2.

12‐LOX kinetics with PUFAs and 15‐oxylipins.

Enzyme + substrate	k cat (s−1)	k M (μM)	k cat/k M (s−1 μM−1)
DGLA	12 ± 0.3	2.5 ± 0.2	4.9 ± 0.3
AA	11 ± 0.2	0.49 ± 0.07	22 ± 3
EPA	9 ± 0.5	1 ± 0.2	8.7 ± 1.2
15‐HpETrE	0.96 ± 0.05	11 ± 1	0.086 ± 0.004
15‐HpETE	1.5 ± 0.05	5.8 ± 0.4	0.26 ± 0.001
15‐HpEPE	0.93 ± 0.06	9.2 ± 1	0.10 ± 0.008

Abbreviations: AA, arachidonic acid; DGLA, dihomo‐γ‐linolenic acid; EPA, eicosapentaenoic acid; PUFAs, polyunsaturated fatty acids; 12‐LOX, 12‐lipoxygenase; 15‐HpEPE, 15(S)‐hydroperoxyeicosapentaenoic acid; 15‐HpETE, 15(S)‐hydroperoxyeicosatetraenoic acid; 15‐HpETrE, 15(S)‐hydroperoxyeicosatrienoic acid.