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. Author manuscript; available in PMC: 2023 Jun 15.
Published in final edited form as: Immunity. 2022 May 25;55(6):982–997.e8. doi: 10.1016/j.immuni.2022.04.016

Figure 7: APCs cross-dress with human HLA molecules.

Figure 7:

A) Monocyte-derived macrophages (MDMs) were differentiated from the blood of an HLA-A*02neg donor and co-cultured with CTV-labeled HLA-A*02pos OCI-Ly8 lymphoma cells for 4 hours with or without an α-CD47 blocking antibody. Representative flow cytometry plots of MDM for acquired OCI-Ly8-derived HLA-A*02 versus CTV (top), CD19 versus CTV (middle), and CD19 versus HLA-A*02 (bottom). B-F) OCI-Ly1 (blue) and OCI-Ly8 (green) tumors were xenografted s.c. into NSG mice (n = 9 each). Representative histograms showing cell surface staining for tumor-derived HLA-I molecules on DCs are shown in (B). Data are quantified as % HLA class I+ among cDC1 (C) and cDC2 (D), as well as normalized MFI of HLA class I for cDC1 (E) and cDC2 (F). Flow cytometry plots in (A) are from one experiment, representative of three independent experiments. Data in (B-F) are pooled from two independent experiments. Statistical significance was determined by one-way ANOVA with post-hoc Tukey’s HSD test.