Figure 88.

i) General procedure for the fabrication and functionalization of the PEG based hydrogels. (1) Precursor solution placed between a methacrylate functional glass slide and PDMS cover. (2) Irradiated under blue light to obtain the crosslinked gel. (3) Gel swollen with acrylate solution and photomask placed on top. (4) Irradiated under UV light to obtain patterned sections. (a) Hydrogel precursor solution. 1 : 1 equivalent of DTT and norbornene moieties. (b) Crosslinks consist of a double thioether linkage. (c) Chemistry of the photopatterned areas. ii)Surface adhesion of NHDF cells. (a) DIC image of NHDF cells after 24 h incubation on the hydrogel surface. (b) Fluorescence confocal image of the photopatterned section of the gel (photopatterned areas appear black). (c) Fluorescence confocal image of NHDF cells stained with propidium iodide. (d) Merged image of (b) and (c). Reproduced, with permission, from reference 447. Copyright 2018 Royal Society of Chemistry.