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. 2023 Jan 28;24:49. doi: 10.1186/s12864-023-09144-4

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 9 — Overexpression of PbLHY delayed flowering in Arabidopsis. (a) Flowering phenotypes of representative control plants and PbLHY-OE lines grown under long-day conditions for six weeks. (b) and (c) The number of days and rosette leaves were recorded in control and PbLHY-OE lines when the first flower was visible. *** p < 0.001 vs. vector control by t test. (d) Expression of PbLHY in control and PbLHY-OE lines. (e-g) Expression of AtGI, AtCO, and AtFT in control and PbLHY-OE lines. Transcript levels were measured by qRT–PCR assay in 12-day-old seedlings under long-day conditions and normalized to AtACT. The highest expression level of each gene was set to 1 for standardized calculation. Error bars represent the standard error. White and black areas indicate light and darkness, respectively