Table 1.
Primary and secondary antibodies used for IHC
| Primary antibody or lectin (manufacturer, catalog #, dilution used) | Secondary antibody (manufacturer, catalog #, dilution used) |
|---|---|
| Human amyloid-β | |
|
Rabbit anti-human β-amyloid (Cell Signaling, 8243S, 1:500) |
Alexa fluor 647-conjugated donkey anti-rabbit (Invitrogen, A-31573,1:500) |
| Smooth muscle cells | |
| Mouse FITC conjugated α-smooth muscle actin (SMA) (Sigma, clone 1A4, F3777, 1:500) | N/A |
| Vasculature | |
| Dylight 488-conjugated L. esculentum lectin (Vector Labs, DL-1174,1:200) | N/A |
| Fibrinogen | |
| Rabbit anti-human fibrinogen, cross-reacts with mouse fibrinogen [48] (Dako, A0080,1:400) | Alexa fluor 568-conjugated donkey anti-rabbit (Invitrogen, A-10042,1:500) |
| PICALM | |
| Goat anti-human PICALM, cross-reacts with mouse PICALM (Santa Cruz, sc-6433, 1:200) | Alexa fluor 568-conjugated donkey anti-goat (Invitrogen, A-11057,1:500) |
| Microglia | |
|
Rabbit anti-mouse Iba1 (Wako, 019-19741; 1:500) |
Alexa Fluor 568–conjugated donkey anti-rabbit (Invitrogen, A-10042, 1:500) |
| Astrocytes | |
| Rabbit anti-bovine GFAP, cross-reacts with mouse GFAP (Dako, Z0334, 1:500) | Alexa Fluor 568–conjugated donkey anti-rabbit (Invitrogen, A-10042, 1:500) |