Table 2.
Primary and secondary antibodies used for Immunoblotting
| Primary antibody (manufacturer, catalog #, dilution used) | Secondary antibody (manufacturer, catalog #, dilution used) |
|---|---|
| PICALM | |
| Rabbit anti-human PICALM, cross-reacts with mouse PICALM (Sigma, HPA019061, 1:1000) | HRP-conjugated donkey anti-rabbit (Invitrogen, A16023,1:3000) |
| β-secretase 1 (BACE1) | |
| Rabbit anti-human BACE1, cross-reacts with mouse BACE1 (Cell Signaling, 5606, 1:1000) | HRP-conjugated donkey anti-rabbit (Invitrogen, A16023,1:3000) |
| Amyloid precursor protein (APP) and APP C-terminal fragments (APP-CTFs) | |
| Rabbit anti-human carboxy terminus of APP, cross-reacts with mouse APP and APP-CTFs (Cell Signaling, 76600S, 1:1000) | HRP-conjugated donkey anti-rabbit (Invitrogen, A16023,1:3000) |
| Neprilysin | |
| Goat anti-mouse neprilysin (R&D systems, AF1126, 1:1000) | HRP-conjugated donkey anti-goat (Invitrogen, A16005, 1:3000) |
| Notch 1, Notch Intracellular Domain (NICD) | |
| Rabbit anti-human N-terminal sequence of the cleaved NICD, cross-reacts with mouse NICD (Millipore Sigma, 07-1232, 1:1000) | HRP-conjugated donkey anti-rabbit (Invitrogen, A16023,1:3000) |
| Insulin degrading enzyme (IDE) | |
| Rabbit anti-rat insulin degrading enzyme (IDE), cross-reacts with mouse IDE (Millipore Sigma, PC730, 1:1000) | HRP-conjugated donkey anti-rabbit (Invitrogen, A16023,1:3000) |
| Loading controls | |
| Rabbit anti-human β-actin, cross-reacts with mouse β-actin (Cell Signaling, 4970S, 1:2000) | HRP-conjugated donkey anti-rabbit (Invitrogen, A16023,1:3000) |
| Rabbit anti-human GAPDH, cross-reacts with mouse GAPDH (Cell Signaling, 2118, 1:2000) | HRP-conjugated donkey anti-rabbit (Invitrogen, A16023,1:3000) |