Figure 2.
Map‐based cloning of the red petal gene R 3 bic . (a) Physical map of the R 3 bic locus. Four genes (ORF1‐4) in the region are indicated by green and grey thick arrows. (b) qRT‐PCR analysis of 4 candidate genes in the petals of LR and LW. ORF1 was strongly expressed in the red petals of LR but was almost not expressed in the white petals of LW. (c) FPKM expression values of candidate genes in the petals of LR and LW. (d) FPKM expression values of ORF1 (GH_A07G0814) and homologous gene of D subgenome (GH_D07G0816) in the petals of LR and LW. (e) Alignment of the ORF sequences of GhTT19 from LW and LR. A base combination of two positions could distinguish GhTT19 LR ‐At, GhTT19 LW ‐At and GhTT19‐Dt using pyrosequencing. (f) The number of base combinations of two positions in 30 positive clones. The genomic DNA of LR and LW were used for cloning. (g) Pyrophosphate sequencing using cDNA of LR petal. Data are presented as means ± SE from at least three biological replicates. **P < 0.01, *P < 0.05 at Student's t‐test.