Table 1. Optimal Buffer Conditions Used in This Study for the Carbocyanine Dye AF647a.
| pH |
||||
|---|---|---|---|---|
| [MEA] (mM) | 6.5 | 7.4 | 8.0 | 8.5 |
| 10 | – | ○ | + | + |
| 50 | – | + | + | ○ |
| 100 | ○ | + | – | – |
| 250 | + | ○ | ||
Performance was rated with + (very good), ○ (decent), and – (bad). For conditions rated +, the buffer contained between 1.5 and 15.6 mM RS– (cf. Figure S6).