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. 2023 Jan 28;14:455. doi: 10.1038/s41467-023-36081-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Segregation of VAMP2-mCherry from ATG9A-EGFP in COS7 cells also expressing synapsin and synaptophysin. Synaptophysin was visualized by immunofluorescence. b The colocalization of the pairs of proteins indicated was calculated by Pearson’s coefficients. Values are means ± SD; N.S., not significant; **p < 0.01 by one-way ANOVA with Tukey’s HSD post hoc test (droplets in 3 different cells were analyzed). c ATG9A is segregated from SV proteins (VAMP2, SCAMP5, Rab3A) but precisely colocalizes with VAC14 in COS7 cells also expressing synaptophysin and synapsin, consistent with proteomics data. d The colocalization of the pairs of proteins indicated was calculated by Pearson’s coefficients. Values are means ± SD; N.S., not significant; **p < 0.01 by one-way ANOVA with Tukey’s HSD post hoc test (droplets in 3 different cells for each condition were analyzed). Source data are provided as a Source Data file. Scale bars, a and c = 10 μm (1 μm for high-magnification images). p-values (b): 3.032 × 10^‒6 [(Syph vs. VAMP2) vs. (Syph vs. ATG9A)], 7.549 × 10^‒6 [(Syph vs. VAMP2) vs. (VAMP2 vs. ATG9A)], 0.07488 [(Syph vs. ATG9A) vs. (VAMP2 vs. ATG9A)]. d 0.3406 (VAMP2 vs. SCAMP5), 0.8552 (VAMP2 vs. Rab3), 5.655 × 10^‒5 (VAMP2 vs. VAC14), 0.7519 (SCAMP5 vs. Rab3A), 1.546 × 10^‒5 (SCAMP5 vs. VAC14), 3.116 × 10^‒5 (Rab3A vs. VAC14).