Fig. 4.

Effect of combined Wee1 inhibition and irradiation on cell survival and cell cycle of EGFRvIII− /+ cells. A DKMGvIII− /+ and BS153vIII− /+ cells were treated with 400 nm adavosertib 2 h prior to irradiation with 6 Gy. Cell cycle assessment was performed 24, 48 and 72 h after treatment. B DKMGvIII− /+ and BS153vIII− /+ cells were treated with 400 nm adavosertib 2 h prior to irradiation with 0, 2, 4 and 6 Gy. Media exchange was performed 24 h after adavosertib treatment. Cell survival was analyzed by colony formation assay. Absolute colony numbers were normalized to the unirradiated controls (n = 3–5; mean with S.E.M; p-values are obtained by using two-tailed Student´s t-test, *p < 0.05)