SIRT1 inhibitor hyperacetylated p53(K-382) in the ovary of F1 offspring. Pregnant dams (F0) were exposed to Cr(VI) (10 ppm) through drinking water from 9.5–14.5 days post-coitum (dpc). Cr(VI) exposed and unexposed dams were injected (i.p.) with EX-527 (50 mg/kg body weight). On postnatal day (PND) 1, F1 offspring were euthanized, and ovaries were processed for Immunohistochemistry (IHC). Representative images of the ovaries are shown from control (A), Cr(VI) (B), EX-527 (C), and Cr(VI)+EX-527 (D). The histogram shows the integrated optical density (IOD) (E). Different lowercase letters (a, b, c, d) indicate significant differences between groups (p<0.05). There are no significant differences between groups marked by the same letter. Each value is mean ± SEM 5 F0 rats (n=5). Arrows indicate germ cells, and the arrowhead indicates a somatic cell.