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. Author manuscript; available in PMC: 2023 Apr 1.
Published in final edited form as: Reprod Toxicol. 2022 Mar 17;109:121–134. doi: 10.1016/j.reprotox.2022.03.007

Fig. 5.

Fig. 5.

SIRT1 inhibitor increased PUMA in the ovary of F1 offspring. Pregnant dams (F0) were exposed to Cr(VI) (10 ppm) through drinking water from 9.5–14.5 days post-coitum (dpc). Cr(VI) exposed and unexposed dams were injected (i.p.) with EX-527 (50 mg/kg body weight). On postnatal day (PND) 1, F1 offspring were euthanized, and ovaries were processed for IHC. Representative images of the ovaries are shown from control (A), Cr(VI) (B), EX-527 (C), and Cr(VI)+EX-527 (D). The histogram shows the integrated optical density (IOD) (E). Different lowercase letters (a, b, c, d) indicate significant differences between groups (p<0.05). Each value is mean ± SEM 5 F0 rats (n=5). Arrow indicates germ cell, and the arrowhead indicates a somatic cell.