Table 1.
Antibody Table (Source, catalog numbers, dilutions, host species and Immunogen)
S.No | Peptide/protein target | Immunogen | Name of Antibody | Manufacturer; catalog # | Species raised in | Dilution used |
---|---|---|---|---|---|---|
1 | Acetyl-p53 | Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human p53 | Rabbit-Anti-p53 (acetyl K382) | Abcam; Ab37318 | Rabbit polyclonal | 1:100 |
2 | PUMA | Synthetic peptide corresponding to C terminal amino acids 180–193 (PLPRGHR APEMEPN) of Human PUMA alpha | Rabbit Anti-PUMA | Abcam; Ab9643 | Rabbit polyclonal | 1:1000 |
3 | BAX | Synthesized peptide derived from human BAX | Rabbit Anti-BAX | Sigma; SAB4502549 | Rabbit polyclonal | 1:1500 |
4 | Cleaved-Caspase-3 | Synthetic peptide corresponding to amino-terminal residues adjacent to (Asp175) in human caspase-3 | Rabbit Anti-Cleaved-Caspase-3 | Cell Signaling; 9661 | Rabbit polyclonal | 1:300 |
5 | BCL2 | N terminal amino acids 1–18 of Human BCL-2; AGRTGYDNREIVMKYIHY | Rabbit Anti-BCL-2 | Abcam; ab7973 | Rabbit polyclonal | 1:250 |
6 | BCL-XL | Synthetic peptide corresponding to residues surrounding Asp61 of human BCL-XL | Rabbit Anti-BCL-XL | Cell Signaling; 2762 | Rabbit polyclonal | 1:200 |
7 | p-AKT | Synthetic phosphopeptide corresponding to residues surrounding Ser473 of mouse AKT. | Rabbit Anti-phospho AKT (Ser473) | Cell Signaling; 3787 | Rabbit monoclonal | 1:50 |
8 | SIRT-1 | Recombinant Human SIRT1 | Mouse Anti-SIRT1 | Abcam; Ab110304 | Mouse monoclonal | 1:100 |