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. 2023 Jan 23;155(4):e202213276. doi: 10.1085/jgp.202213276

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© 2023 Nelson et al.

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Figure 3. — Event localizations and distributions. (A) Top: Combined fluorescence image showing anti-myomesin (red) and BODIPY-ATP (green), combined from 100 s of acquisition. Bottom: X-Y plot of resulting localization data for the same interval. (B) Scatterplot showing distance from M-line and lifetime for each localized event. WT (black), ΔC0C1f (green), and t/t (red). (C) Histograms showing event frequency as a function of distance from the M-line. (D) Fitting the double-exponential model within a sliding window shows a gradient in SRX abundance for all three mouse models. Linear regressions were fitted through data in the bare and P-zones and separately through the C- and D-zones for all three models. Fit parameters are reported in Table S1. (E) Fractions of SRX/DRX events for events when binned by zone with lifetime distribution plots for these results in Fig. S1. * denotes P < 0.05 by KS test. Error bars denote the standard error of the fit. The C-zones of both t/t and ΔC0C1f were significantly different than WT (P = 0.02 and P = 0.01, respectively), as were the D-zones (P = 0.0007 and P = 0.04). D-zone event distributions were significantly different from the C-zone for WT (P = 0.04), t/t (P = 0.04), and ΔC0C1f (P = 0.048). WT events are combined from 13 technical replicates taken from 4 biological replicates. t/t events are combined from 11 technical replicates taken from 4 biological replicates. ΔC0C1f events are combined from 7 technical replicates taken from 3 biological replicates.