Fig. 2.

Co-housing with 6 M mice exacerbated psoriasis-like skin phenotype in and changed the intestinal microbiota composition of, 2 M mice. a Macroscopic characteristics of the ears of Sep-2M Co-2M and 6 M mice in 0 week and 4 weeks. b PASI score of ears in Sep-2M Co-2M and 6 M mice from 0 week to 4 weeks. c Pathological score of ear sections using the Baker scoring system. d Average epidermal thickness. e Analysis of Th17 cells (CD4⁺ IL-17⁺) by flow cytometry in DLNs. f Percentage of Th17 cells in CD4⁺ cells. g Relative mRNA expression of IL-17A in the ears. h Analysis of Th17 cells (CD4⁺ IL-17⁺) by flow cytometry in ears. i Number of Th17 cells in ears. j Relative mRNA expression of IL-23 in the ears. k PcoA of unweighted UniFrac distance based on 16s rDNA profiling of feces from Sep-2M Co-2M and 6 M mice. l Results of LEfSe analysis showing bacteria, at the lowest taxonomic level, that were significantly different in abundance in Sep-2M and Co-2M mice. m, n Fecal contents of Sep-2M and Co-2M mice were analyzed for Prevotella and Parabacteroides distasonis colonization by qPCR. o, p Representative fluorescence in situ hybridization for Prevotella (Prv392) and Parabacteroides distasonis (PD) in the colon. Data presented as mean ± SD on relevant graphs. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.005. A two-tailed Student’s t-test was used in (m) and (n), one-way ANOVA was used for comparing the rest of the groups. (n = 6) in (a–j). Sep-2M (n = 8), Co-2M (n = 7) and 6 M (n = 7) in (g, h). (n = 6) in o, p