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. 2023 Jan 30;8:40. doi: 10.1038/s41392-022-01219-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Co-housing with 6 M mice changed the intestinal metabolite composition and metabolic pathways of 2 M mice. a Principal components analysis (PCA) of fecal metabolites from Sep-2M Co-2M and 6 M mice. b Heatmap of metabolites identified in fecal samples. c Volcano plot of metabolites identified in fecal samples. Volcano plots are depicted with the fold change of each metabolite and the q value was calculated by performing a t-test and corrected by the false discovery rate. d, e Pathway enrich analysis of metabolites from Sep-2M and Sep-6M mice, and Sep-2M and Co-2M mice. f, g Relative abundance of linoleic acid and oleic acid in fecal metabolites. Data presented as mean ± SD on relevant graphs. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.005 (one-way ANOVA). Sep-2M (n = 8), Co-2M (n = 8), and 6 M (n = 7)