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. 2023 Jan 16;10:993165. doi: 10.3389/fped.2022.993165

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© 2023 Zhang, Gu, Zhang, Xu, Wei, Chen, Shi, Sun, Zhou and Zhao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Histochemical and immunohistochemical staining of muscle biopsies (×200). Following HE staining (A), different sizes of muscle fibers were observed, and degeneration, necrosis, and regeneration of the muscle fibers was evident as well as the deposition of amorphous materials. Some muscle fiber staining was significantly darker than the surrounding myofibrils according to modified Gömöri trichrome staining (B). Fluorescence antibody staining of desmin, α-crystallin (C), and myotilin (D) revealed high expression levels of these proteins in the sediment.