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. 2023 Jan 24;11(1):e005518. doi: 10.1136/jitc-2022-005518

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© Author(s) (or their employer(s)) 2023. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

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BCG instillations induce H3K4me3 modifications in monocytes. Results of the ChIP-PCR analysis for H3K4me3 modifications. H3K4me3 signal was calculated as %input and the fold change (of %input) is shown for pre-BCG7 and pre-BCG10 compared with pre-BCG1. The fold change is shown for six genomic regions in the promoter of TNF, IL1B and IL6 (the primers are displayed in online supplemental table 13). (A) TNF region 1, (B) TNF region 2, (C) IL1B region 1, (D) IL1B region 2, (E) IL6 region 1, (F) IL6 region 2. Two-tailed matched-pairs Wilcoxon signed-rank test was used to determine statistical significance in between time points. Statistical significance was accepted at p<0.05 and indicated as follows: *p<0.05, **p<0.01, ***p<0.001 ****p<0.0001. ChIP, chromatin immuno precipitation.