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. 2023 Jan 16;13:1079710. doi: 10.3389/fmicb.2022.1079710

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Copyright © 2023 Srimahaeak, Thongdee, Chittrakanwong, Atichartpongkul, Jaroensuk, Phatinuwat, Phaonakrop, Jaresitthikunchai, Roytrakul, Mongkolsuk and Fuangthong.

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gidA is needed for the full virulence of P. aeruginosa. (A) Cell viability of the RAW264.7 macrophage cell line after treatment with the wild-type, gidA mutant, and complemented cells for 24 h. Cell viability at each timepoint was examined and compared with that of the untreated cells. (B) L4 stage larvae of C. elegans were added to the lawn of the wild-type, gidA mutant, and complemented cells. Live and dead worms were counted at time intervals for 3 days. The wild type and gidA mutant carried the pBBR1MCS-4 plasmid as a vector control. The data represent the mean ± SD values of three biological replicates. The statistical analysis was performed using Graphpad Prism (GraphPad Software). Asterisks denote a significant difference in the one-way ANOVA (* p ≤ 0.05).