Table 1.
Strains and plasmids used in this study.
| Strain/plasmid | Characteristic | Reference/source |
|---|---|---|
| P. aeruginosa PA14 | ||
| Wild type | P. aeruginosa UCBPP-PA14 wild-type strain | ATCC |
| gidA mutant | gidA::pKNOCK-Gm,Gmr | This study |
| gidA complemented | gidA mutant harboring pBBR1MCS-4/gidA_FL | This study |
| E. coli | ||
| BW20767 | leu-63::IS10recA1creC510 hsdR17 endA1zbf-5uidA(∆MluI)::pir+thi RP4-2-tet::Mu-1kan::Tn7 | Metcalf et al., 1996 |
| DH5α | λ− ϕ80dlacZ∆M15 recA1 endA1gyrA96thi-1hsdR17(rK−mK+) supE44relA1deoR ∆(lacZYA-argF)U169 | Stratagene Inc. (United States) |
| DE3 (BL21) | E. coli B F- dcm ompT hsdS (rB-mB-) galλ (DE3) | Stratagene Inc. (United States) |
| Plasmids | ||
| pKNOCK-Gm | Suicide plasmid for insertional inactivation of gidA | Alexeyev, 1999 |
| pKN-gidA | pKNOCK-Gm with internal 215-bp fragment of gidA | This study |
| pBBR1MCS-4 | Broad-host-range plasmid | Kovach et al., 1995 |
| pBB-gidA-FL | pBBR1MCS-4 expressing PA14 GidA | This study |
| pBB-katB-6Xhis | pBBR1MCS-4 expressing His-tagged KatB | This study |