Figure 4.

Clonogenic assay. TMA inhibits colony formation of (A) HCT116 and (B) HT29 cells. Evaluation of colony forming ability in HCT116 and HT29 cell line exposed to various concentrations of TMA for 48 h. TMA inhibits the colony forming ability of HCT116 in a concentration dependent manner. Results were plotted as no. of colonies formed after 14 days (shown right to the colony images). Negative control cells were treated with cell culture water only. Difference of average values between untreated and treated cells were tested using paired student’s t-test. All tested subjects showed significant decrease in viability while 10 mM TMA proved most cytotoxic to cells. The results (mean±SEM) were obtained from 3 independent experiments. *indicates significantly different values compared to control group (P<0.05).