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. 2023 Jan 16;13:1026304. doi: 10.3389/fimmu.2022.1026304

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Copyright © 2023 Toscano, Elst, Van Gasse, Beyens, van der Poorten, Bridts, Mertens, Van Houdt, Hagendorens, Van Remoortel, Timmermans, Ebo and Sabato

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MRGPRX2 expression in whole blood vs purified basophils and MRGPRX2 functionality in purified basophils. (A) Effect on MRGPRX2 expression on the surface membrane of basophils from HCs after purification using magnetic beads (n=12). **** p ≤ 0.0001; paired Student’s t-tests. (B) Membrane expression of CD63 on purified basophils from HCs resuspended in RPMI medium, primed with IL-3 (2 ng/mL and 10 ng/mL) and subsequently stimulated with SP for 3 and 20 minutes (n=6). * p ≤ 0.05; ** p ≤ 0.01 SP 300 µmol/L after priming with IL-3 compared to buffer after priming with IL-3; paired Student’s t-tests. (C) Membrane expression of CD63 on purified basophils from the same HCs as in Figure 6B resuspended in RPMI medium, without priming with IL-3, and stimulated with SP for 3 and 20 minutes (n=6). HCs = healthy controls. SP: substance P.