FIGURE 3.

Food and Drug Administration (FDA)-approved drugs increase forskolin-induced swelling (FIS) in homozygous W1282X/W1282X patient-derived intestinal organoids (PDIOs). a) Schematic of workflow of FDA-approved drug library testing on W1282X/W1282X PDIOs. b) Z’-factor for each 384-well plate for the four PDIOs. F508del/S1251N PDIOs treated with VX-770 served as positive control. c) Area under the curve (AUC) values of the top 17 compound combinations, means of the triplicate experiments of all PDIOs are shown with the different PDIOs indicated by the four different coloured dots. d) Pictures of the PDIO line 1, of the primary screen, for the top five compound combinations and the DMSO control (t=0 h and t=3 h).