TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Genotype or description | Source or reference |
|---|---|---|
| Strains | ||
| Y. pseudotuberculosis | ||
| YPIIIpYV | Wild type | Stanley Falkow |
| MT2294 | dam::Kn + pTP166-Cm | This work |
| MT2357 | DUP[φ(dam::Kn)∗pCVD442∗(dam+)] | This work |
| MT2358 | DUP[φ(dam::Kn)∗pCVD442∗(dam+)] pWKS30-Tc + Y. pseudotuberculosis dam+ | This work |
| MT2359 | DUP[φ(dam::Kn)∗pCVD442∗(dam+)] pWKS30-Tc + E. coli dam+ | This work |
| MT2360 | DUP[φ(dam::Kn)∗pCVD442∗(dam+)] pWKS30-Tc | This work |
| V. cholerae | ||
| O395 | Wild type; classical | John Mekalanos |
| MT2284 | dam::Kn pWKS30-Tc + E. coli dam+ | This work |
| MT2361 | DUP[φ(dam::Kn)∗pCVD442∗(dam+)] | This work |
| MT2362 | DUP[φ(dam::Kn)∗pCVD442∗(dam+)] pWKS30-Tc + V. cholerae dam+ | This work |
| MT2363 | DUP[φ(dam::Kn)∗pCVD442∗(dam+)] pWKS30-Tc + E. coli dam+ | This work |
| MT2364 | DUP[φ(dam::Kn)∗pCVD442∗(dam+)] pWKS30-Tc | This work |
| S. enterica serovar Typhimurium | ||
| MT2188 | damΔ232 | 20 |
| Plasmids | ||
| pTP166-Cm | E. coli dam under tac promoter control; chloramphenicol-resistant (Aps) derivative of pTP166 | 29; this work |
| pWKS30-Tc | Tetracycline-resistant (Aps) derivative of pWKS30 | 43; this work |