FIGURE 2.

Overexpression of miR‐204‐3p attenuates HKCs' response to high‐glucose conditions. High glucose‐treated keratinocytes transfected with miR‐NC or miR‐204‐3p for 48 hours. (A and B) Apoptosis was analysed using flow cytometry (the NG group and HG group were the negative controls in the transfection experiment). (C) Cell proliferation was monitored by CCK‐8 assay. (D) Cell migration was tested using Transwell inserts. (E) The expression of miR‐204‐3p was detected by qRT‐PCR. (F‐K) The protein expression of TGF‐β, MMP‐9, Bcl‐2, Bax, and cleaved caspase‐3 was detected by western blotting; *P < .05 vs the NG group; ^# P < .05 vs the HG + miR‐NC group; n = 3 in each group