FIGURE 4.

Functional proof for miR‐204 binding to the KLF6 UTR. (A) The putative binding sites in the 3′‐UTR of KLF6 and miR‐204‐3p predicted by online software. (B) The luciferase reporter assay was performed in HKCs with the wild‐type (WT) sequence of KLF6 or its mutant‐type (MUT) sequence and cotransfected with miR‐NC, miR‐204‐3p, anti‐miR‐NC or anti‐miR‐204‐3p in the luciferase reporter plasmids. (C and D) The protein expression of KLF6 was detected in HKCs transfected with miR‐204‐3p or anti‐miR‐204‐3p. *P < .05 vs the miR‐NC group; ^# P < .05 vs the anti‐miR‐NC group; n = 3 in each group