FIG. 2.

Detection of qlp42 by PCR using primers bpNF and bpNR. An amplified fragment of 1,395 bp suggested conservation among pathogenic L. interrogans serovars. Only a faint signal was amplified from lanes B and G. Lane M; DNA ladder (size indicated in base pairs); lane −, negative control; lane Bb, B. burgdorferi; lane Lb, nonpathogenic L. biflexa; lane B, L. interrogans serovar bratislava; lane C, serovar canicola; lane G, serovar grippotyphosa; lane H, serovar hardjo; lane I, serogroup Icterohaemorrhagiae serovar copenhageni; lane P, serovar pomona; lane J, JEN4; lane +, positive control.