Detection of hsp15 among pathogenic L. interrogans serovars by PCR using primers LepCFXho and LepCRBam. Lane M, DNA ladder (size indicated in base pairs); lane −, negative control; lane Bb, B. burgdorferi; lane Lb, nonpathogenic L. biflexa; lane B, L. interrogans serovar bratislava; lane C, serovar canicola; lane G, serovar grippotyphosa; lane H, serovar hardjo; lane I, serogroup Icterohaemorrhagiae serovar copenhageni; lane P, serovar pomona; lane J, positive control JEN4. The amplified fragments were approximately 468 bp.