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proTrh expression in the PVN of fasting THAI mice. (A, B) 24 hours fasting, (C, D) 48 hours' fasting. (A, C) Representative dark-field micrographs of proTrh in situ hybridization at the mid-level PVN of fasting YA. (B) Quantification of the proTrh mRNA hybridization signal of (A), (D) Quantification of the proTrh mRNA hybridization signal of (C). n(24 hours) = 10–11/group; n(48 hours) = 7–8/group; figure shows Tukey Box Plots, α = 0.05; **p < 0.01. Scale bar: 100 μm.
