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. 2023 Jan 30;42:35. doi: 10.1186/s13046-022-02592-y

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Transcriptomic profiles of lean and obese bASCs adjacent to breast cancers. A-G Total RNAs were extracted from each sample of bASC subgroups (ln-dT, ln-aT, ob-dT and ob-aT, 5 samples for each subgroup) for transcriptome analysis. A and B Heatmap of significantly differentially expressed genes of ln-dT vs. ln-aT bASCs (A) and ob-dT vs. ob-aT (B). Gene expression was analyzed using DESeq2 R package. Genes with a p-value < 0.01, and a fold change greater than 2 (red color code) and below − 2 (blue color code), respectively, were included. C and D Volcano plots showing the adjusted p-value (adj. p > 0.05) for genes differentially expressed between bASCs close to and distant to the breast cancers in lean (C) and obese (p > 0.05) (D) bASCs. Upregulated genes are depicted in red color, downregulated in green, and non-changed genes in blue (adjusted p-value > 0.05). E and F Significantly enriched KEGG pathways (E) and GO pathways (F) are presented for ln-aT bASCs compared to ln-dT bASCs. For each KEGG or GO pathway, the bar shows the adjusted p-value. The numbers (n) behind the pathway names indicate deregulated genes. G Violin plots present selected myCAF/iCAF genes differentially expressed. Values reflect the log expression levels of genes from the RNA-seq data. H Heatmap depicts significantly differentially expressed cytokine/chemokine genes in four bASCs subgroups. These genes have a fold change greater than 2 (red color code) or below − 2 (blue color code). Genes in (A-F) have an adjusted p-value of ≤0.05 and genes shown in (G) have an adjusted p-value of ≤0.05, at least for one condition (ln-aT or ob-aT)