Fig. 4.

bASCs adjacent to breast cancer cells have increased gene expression and protein secretion of cancer promoting cytokines. A Relative gene levels of IL6, IL8, CXCL1, CXCL2, CXCL3, CCL2, VEGFC, FGF1, FGF2, and LIF are shown for bASC subgroups (ln-dT, ln-aT, ob-dT and ob-aT). The results are from three independent experiments and presented as bar graphs with mean ± SEM. B-E ELISA assays were performed with conditioned media from bASCs subgroups and the levels of cytokines IL6 and IL8 (B), chemokines CXCL1–3 and CCL2 (C), growth factors VEGFC and FGF1/2 (D), and inflammatory cancer-associated cytokines CSF3, CXCL10, IL1β and LIF (E) were analyzed. The results are from three independent experiments and presented as scatter bar graphs with mean ± SEM. Student’s t test was used. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001