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. 2023 Jan 30;42:35. doi: 10.1186/s13046-022-02592-y

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Transcriptome profiles of triple positive BT474 cells directly co-cultured with bASCs. A-F BT474 cells cultured for 14 days alone or in direct co-culture with different bASCs (ln/ob-dT/aT) were sorted for RNA-seq analysis (n = 3). The gene expression was analyzed using DESeq2 R package. A Volcano plots show the adjusted p-value for genes differentially expressed between control BT474 cells and BT474 cells directly co-cultured with ln-aT bASCs (1^st volcano plot), ln-dT bASCs (2^nd volcano plot), ob-aT bASCs (3^rd volcano plot) and ob-dT bASCs (4^th volcano plot). B and C GO enrichment analysis of RNA-seq data with a p-value ≤0.05. Gene count: the number of genes that are deregulated in the pathway. Gene ratio: ratio of the number of target genes divided by the number of all genes in each pathway. Significance is color coded as indicated (high p-value, red; low p-value, blue). D-F Heatmaps of deregulated genes involved in motility and migration (D), DNA repair and apoptosis (E), and cell cycle regulation (F). A fold change of ≥2 (red color code) and ≤ − 2 (blue color code) are shown. Included genes have a p-adjusted value of ≤0.05 for at least one condition (ln-aT or ob-aT)