Skip to main content
. 2001 Dec;69(12):7642–7651. doi: 10.1128/IAI.69.12.7642-7651.2001

FIG. 4.

FIG. 4

Western and colony immunoblots of clonal variants derived from M. penetrans isolate 75 showing phase-variable expression of P34B and P38. (A) SDS-PAGE analysis of LAMPs from two selected clones of M. penetrans 75. Clone K1 was positive with P38 (MAb G9) and negative with MAb D10, and clone 2 reacted positively with MAb D10 (P34B) and negatively with MAb G9. (B) Clones K1 and K2 were grown on agar plates, and colony immunoblots were labeled with MAb D10 or MAb G9. Staining of clone K1 colonies with MAb D10 revealed the presence of colonies among the P34B negative population that have reverted to the P34B-positive phenotype (arrow p). Colonies generated with clone K2 are predominantly positive for P34B, though a few present a negative (arrow n) and sectoring (arrow s) staining. Staining of colonies generated with clone K1 with MAb G9 showed predominantly the P38-positive phenotype. Colonies of clone K2 were negative. Bar, 100 μm.