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. Author manuscript; available in PMC: 2023 Jul 12.
Published in final edited form as: Immunity. 2022 Jun 13;55(7):1173–1184.e7. doi: 10.1016/j.immuni.2022.05.010

Figure 3: Foxp3 is dispensable for lineage commitment of microbiota-dependent pTreg cells.

Figure 3:

A-B) Flow cytometry of CD4 T cells from spleen and colonic lamina propria (LP) of female Foxp3DTR-GFP/loxp-Thy1.1-STOP-loxp-GFP mice. Showing one of two independent experiments with 5–6 mice per group each. P-values from paired t-test (****:p<0.0001). C) RNA-seq of DTR-GFP+ wild-type Treg cells and Thy1.1+ reporter-null cells from the LP of female Foxp3DTR-GFP/loxp-Thy1.1-STOP-loxp-GFP mice (left). Comparison with published dataset of colonic RORγt+ vs RORγt− colonic Treg cells (right). D) Differential expression of the pTreg transcriptional signature by LP Treg cells and Foxp3 reporter-null cells. P-values from one-sided Kolmogorov-Smirnov test comparing red and blue distributions to the background distribution in black. E) Flow cytometry of splenic (top) and LP (bottom) CD4 T cells from female Foxp3ΔEGFPiCre/wtR26lsl-tdTomato mice maintained on antibiotic-containing or regular drinking water (SPF) for one month. Pooled data from two independent experiments with n=4 or n=6 mice per group. P-value from unpaired t-test (***:p<0.0001).