Table 3.

K-CLIP reaction conditions for p53 and subsequent Western blot analysis ^*

	Negative Control #1	Negative Control #2	Crosslinking Reaction
HEK293 lysate (500 μg)	13.5 μL	13.5 μL	13.5 μL
Kinase Buffer (1X)	4 μL	4 μL	4 μL
ATP (5 mM)	4 μL	-	-
ATP-arylazide (5 mM)	-	4 μL	4 μL
Water	18.5 μL	18.5 μL	18.5 μL
UV	+	-	+

^*For Basic Protocol 2, the stock concentrations are 37 mg/mL HEK293 lysate, 50 mM ATP-arylazide, and 50 mM ATP. The HEK293 lysate contains kinases and the p53 substrate endogenously.