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Setup for Immunoprecipitation of p53 samples*
| Negative Control #1 | Negative Control #2 | Crosslinking Reaction | IP Negative Control #1 | Optional IP Negative Control #2 | |
|---|---|---|---|---|---|
| Basic Protocol 1 Kinase reaction samples | 40 μL | 40 μL | 40 μL | 40 μL | - |
| p53 Antibody (1:1000) | 10 μL | 10 μL | 10 μL | - | 10 μL |
| Agarose beads | 20 μL | 20 μL | 20 μL | 20 μL | 20 μL |
For the p53 example, method A was used for immunoprecipitation.
