Table 5.

The number of M₁ plant progenies, progenies segregating in M₂ population, mutated plant percent, mutagenic effectiveness, and efficiency induced by caffeine and sodium azide (SA).

Mutagen treatment	Number of seeds sown in M1	Number of seeds germinated in M1	Number of fertile M1 plant progenies	Number of seeds sown in M2	Number of seeds germinated in M2	Number of fertile M2 plants	Number of M2 mutants	Mutated plant (%) Mp	Biological damage in M1 generation (% meiotic abnormalities) Me	Mutagenic effectiveness	Mutagenic efficiency (Mp/Me)
Control	100	91	89	100	92	91	–		–	–	–
Caffeine (%)
0.2	100	85	82	100	87	85	5	6.09	2.71	3.38	2.25
0.4	100	82	80	100	84	83	9	11.25	5.26	3.13	2.14
0.6	100	76	74	100	78	75	10	13.51	9.28	2.50	1.45
0.8	100	71	68	100	74	70	12	17.65	13.28	2.45	1.33
1.0	100	65	62	100	68	64	13	20.97	17.71	2.33	1.18
SA (%)
0.2	100	83	81	100	85	83	8	9.87	5.63	5.48	1.75
0.4	100	79	77	100	81	78	9	11.68	7.66	3.24	1.52
0.6	100	72	69	100	75	72	11	15.94	13.21	2.95	1.21
0.8	100	66	64	100	67	65	12	18.75	18.28	2.60	1.03
1.0	100	61	58	100	63	60	14	24.14	23.13	2.68	1.04