. Author manuscript; available in PMC: 2023 Oct 1.

Published in final edited form as: Curr Protoc. 2022 Oct;2(10):e569. doi: 10.1002/cpz1.569

Table 1.

Media for Each Cell Line and Harvesting Conditions (when Cells Are Harvested Directly from Original Vial from ATCC)

S.no.	Cell lines	Culture medium	Conditions	Growth rate	Confluency
1.	BT-474	Roswell Park Memorial Institute 1640 medium (RPMI-1640)	37°C, 5% CO₂	2–3 days	70%−80%
2.	MCF-7	RPMI-1640	37°C, 5% CO₂	3–5 days	70%−80 %
3.	Calu-3	Eagle’s Minimum Essential Medium (EMEM)	37°C, 5% CO2.	5–7 days	70%−80%
4.	A549	RPMI-1640	37°C, 5% CO₂	2–3 days	70%−80%