Fig. 1.

ISG15 pathway knockdown reverses the transformed phenotype of PDAC cells. a Clonogenic assay. Growth of wild type (left), clonal ISG15 (middle), and UbcH8 (right) knockdown cells are shown after 4 days. b Quantitation of clonogenic assay. Colony area % and colony intensity % was measured. (*p value < 0.002, N = 6). c A MTT proliferation assay showing significant decreased cell proliferation with clonal ISG15 and UbcH8 knockdown cells compared to wild type cells (*p value < 0.0001, N = 12)