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Repeat mechanical testing after 3 wk culture increased engineered heart tissue (EHT) force. A: relationship of EHT force (normalized) to EHT age. First, EHT mechanical testing was at 3 wk (n = 6, same EHT as Fig. 6B), with retesting after 1 day or 1 wk (P value for linear regression). B: effect of both EHT age and repeat testing on EHT force (data from Fig. 6B and Fig. 7A) (P values for unpaired t tests).
