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. 2023 Jan 23;6(1):52–67. doi: 10.1038/s41929-022-00904-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 2 — a. A representative micrograph with example gyrase particles indicated. b. A selection of 2D classes, box size in angstroms indicated. c. FSC curve for the highest-resolution reconstruction as outputted by cryoSPARC. d. Euler angle distribution as output by cryoSPARC. e. Processing scheme (see Methods for description). f. Local resolution maps contoured at two different levels (5σ; 15σ) to illustrate resolution distribution from >2.5 Å next to the DNA and compound to <4 Å at the ends of the TOPRIM insert. g. Map-to-model curve.