Extended Data Fig. 8. Trem2 deficiency exacerbates cardiac dysfunction in sepsis, related to Fig. 6.
a-i, WT and Trem2-/- mice were subjected to CLP and cardiac function was examined at steady state (SS) and 3, 7 and 21 days post-CLP. Graph showing FS% (a); CO (b) measured by echocardiography; Graph showing the levels of LDH in the serum (c); Graphs showing mRNA levels of Anp (d), Bnp (e), Tnfα (f), Il-1β (g), Ccl2 (h) and Il-6 (i) in the heart tissue lysates (a-b, n = 7–8 mice; c, n = 6-8 mice; d-i, n = 6-7 mice for each treatment). j, Study protocol of bone marrow transplantation (BMT) from WT and Trem2-/- donors (CD45.2 background) into WT recipients (CD45.1 background). k, Representative contour plots of CD45.1 and CD45.2 expressions on gated CD45+CD11b+F4/80+CD163+RETNLA+ Mac1 subset in recipient mice 1, 3, 5 and 8 weeks after BMT. l, Graph showing percentages of CD45.2+ cells in CD11b+F4/80+CD163+RETNLA+ Mac1 subset after BMT (n = 4-7 mice for each group).m, Representative contour plots of TREM2 expression on gated CD45+CD11b+F4/80+CD163+ macrophages in hearts of both WT→WT chimeras (Trem2+/+Ch) and Trem2-/-→WT chimeras (Trem2-/-Ch) 8 weeks after BMT. Graph showing Trem2 mRNA levels in heart tissue lysates (n = 6 mice for each group). n, Graphs showing mRNA levels of Anp and Bnp in the heart tissue lysates 7 days post-CLP (n = 11 mice for each group). o, Graphs showing mRNA levels of Tnfα, Il1β, Ccl2 and Il6 in the heart tissue lysates 7 days post-CLP (n = 11 mice for each group). Each symbol represents one animal. All data are presented as mean ± SEM. Two-sided P values were determined by Mann-Whitney U test (a-i,m-o) or by Kruskal-Wallis with Dunn’s multiple comparison test (l). Results represent at least four (a-i,l) and three (m-o) independent experiments.