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. 2023 Jan 12;5(1):129–146. doi: 10.1038/s42255-022-00715-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, Dot plots showing the top 20 biological processes for the upregulated DEGs of Mac1 analyzed by GO. Red arrows indicate endocytosis-related pathways. b, UMAP plots and violin plots showing the expression of Trem2 in the monocyte-macrophage compartment. c, Representative immunofluorescence images of CD163 (green), TREM2 (red) and nuclei (blue) in cardiac tissue from WT mice at SS (n = 5) and 7 d after CLP (n = 5). The dotted lines indicate CD163⁺TREM2⁺ macrophages. Scale bars, 20 μm. d, Representative histograms of flow cytometric analysis of TREM2 expression in cardiac CD45⁺CD11b⁺F4/80⁺CD163⁺RETNLA⁺ and CD45⁺CD11b⁺F4/80⁺(non-CD163⁺RETNLA⁺) macrophages from WT mice at SS and 7 d after CLP. e, UMAP plots showing cell clustering results corresponding to Fig. 2a for a total of 13,405 monocytes-macrophages in WT and Trem2 knockout (KO) hearts at 7 d after CLP. f, Cluster distribution within the monocyte-macrophage subsets in WT and Trem2-KO hearts at 7 d after CLP. g, Percentages and absolute numbers of CD163⁺RETNLA⁺ macrophages analyzed by flow cytometry from hearts of WT (n = 6) and Trem2-KO (n = 6) mice at 7 d after CLP. h, Representative contour plots showing the expression of Ki67 on gated CD45⁺CD11b⁺F4/80⁺CD163⁺RETNLA⁺ macrophages and graph showing percentages of Ki67-expressing cells in WT (n = 5–6) and Trem2-KO (n = 6–7) hearts at SS and 3 d after CLP. Every symbol represents a mouse (g,h). Data are presented as mean ± s.e.m.; two-sided P values were determined by unpaired t-test (g) and one-way ANOVA with Sidak’s multiple comparisons test (h). Results represent three independent experiments (c,d,g,h). See also Extended Data Fig. 5.

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